Tag: pipette

A pipette is a device for quantitatively transferring liquid. A pipette is generally used to remove small or trace amounts of liquid in analytical testing studies. Forward pipetting and reverse pipetting are the 2 methods of piprtting. Before pipetting, to make sure the pipette, nozzle, and liquid are at the same temperature. In this article we will describe these two pipetting methods in detail.

Pipette is a device for quantitative transfer of liquid, which is widely used in analysis and testing, chemistry and other fields and clinical diagnostic laboratories. We commonly use manual adjustable pipette and electric points, specific also according to the different volume of liquid removal has many optional specifications. We not only need to know how to use the pipette, the most important thing is to know what to pay attention to when using it. In this article, ATO automation will give you a detailed introduction.

Pipette also known as the single line pipette, is used to accurately remove a certain amount of liquid, is a common glass instrument in the laboratory daily inspection. In the content determination, the quality of pipette and the method of use have a great influence on the accuracy of the final data. In order to ensure the accuracy of the liquid volume and the reliability of the experimental results, we can pay attention to these aspects. In this article, we mainly introduce the key points when using pipette.

In order to ensure the accuracy and precision of the pipette, regular maintenance should be carried out according to the specific use situation. The pipette should be cleaned especially after removal of corrosive solvents. By simple cleaning and maintenance, the service life of the pipette can be properly extended. In this article, we will introduce the methods of pipette cleaning.

The fourth step is to check the piston system. After disassembling the piston, it is required to check if the piston is polluted, clean the piston, smear lubrication oil, re-install the piston, and recheck if the pipetting volume is still low. If the problem can not be solved, please deliver to the supplier. If there are serious problems such as aging of the sealing ring or rust of the piston, please also send it to the supplier for handling. Generally, users are not recommended to replace the accessories after purchasing the accessories from the supplier.

Check whether the sealing device and piston are polluted. The piston should be glossy and free of corrosive rust. If it is contaminated, clean it with distilled water or isopropyl alcohol, and then wipe it with a non-woven cloth. At the same time, note that before re-installing, check whether the inner wall of the handle is clean and ensure that all parts are completely dry.

The pipette is a single-channel micropipette that is commonly used in biological and chemical laboratories to move liquids with a small volume, and it is a measuring tool for transferring liquid from the original container to another container within a certain range. The design of the pipette fully conforms to the ergonomic principle, and is characterized by easy disassemble and maintenance. The pipette has five modes: Pipetting mode, sample mixing mode, reverse aspiration mode, electrophoresis sampling mode, and graded pipetting mode. The pipette can be applied to liquids such as high viscosity liquids, high vapor pressure liquids, and foaming liquids. Pipettes are widely used in clinical diagnostic laboratories, biotechnology laboratories, pharmaceutical and chemical laboratories, environmental laboratories, and food laboratories.

Before pipetting, it is required to make sure that the pipette, pipette tip and liquid are at the same temperature. In sucking liquid, the pipette must be kept in an upright position and the pipette tip shall be inserted 2-3 mm below the liquid surface. Before sucking the liquid, it is recommended to suck and release the liquid for several times to wet the suction nozzle (especially when it is going to suck liquid with a viscosity or when the density is different from water). At this time, two pipetting methods can be used: 

The first is the forward pipetting method. Press the button to the first stop with the thumb and then slowly release the button to the original point(suck the liquid of the fixed volume), and then press the button to the first stop point to drain out the liquid, wait for a while, continue to press the button to the second stop point to blow out the residual liquid, and release the button in the end.